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Gut and Liver is an international journal of gastroenterology, focusing on the gastrointestinal tract, liver, biliary tree, pancreas, motility, and neurogastroenterology. Gut atnd Liver delivers up-to-date, authoritative papers on both clinical and research-based topics in gastroenterology. The Journal publishes original articles, case reports, brief communications, letters to the editor and invited review articles in the field of gastroenterology. The Journal is operated by internationally renowned editorial boards and designed to provide a global opportunity to promote academic developments in the field of gastroenterology and hepatology. +MORE
Yong Chan Lee |
Professor of Medicine Director, Gastrointestinal Research Laboratory Veterans Affairs Medical Center, Univ. California San Francisco San Francisco, USA |
Jong Pil Im | Seoul National University College of Medicine, Seoul, Korea |
Robert S. Bresalier | University of Texas M. D. Anderson Cancer Center, Houston, USA |
Steven H. Itzkowitz | Mount Sinai Medical Center, NY, USA |
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Shang-Chin Huang1 , Hau-Jyun Su1
, Jia-Horng Kao1,2,3
, Tai-Chung Tseng1,3
, Hung-Chih Yang1,3
, Tung-Hung Su1,3
, Pei-Jer Chen1,2,3
, Chun-Jen Liu1,2,3
Correspondence to: Chun-Jen Liu
ORCID https://orcid.org/0000-0002-6202-0993
E-mail cjliu@ntu.edu.tw
Shang-Chin Huang and Hau-Jyun Su contributed equally to this work as first authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gut Liver 2021;15(3):451-458. https://doi.org/10.5009/gnl20218
Published online January 13, 2021, Published date May 15, 2021
Copyright © Gut and Liver.
Background/Aims: Fatty liver disease is defined as a cluster of diseases with heterogeneous etiologies, and its definition continues to evolve. The novel conceptional criteria for metabolic dysfunction-associated fatty liver disease (MAFLD) were proposed in 2020 to avoid the exclusion of a certain subpopulation, but their evaluations have been limited. We aimed to examine and compare the clinical as well as histologic features of MAFLD versus nonalcoholic fatty liver disease (NAFLD) in patients with biopsy-proven hepatic steatosis.
Methods: From January 2009 to December 2019, 175 patients with histology-proven hepatic steatosis and 10 with cryptogenic cirrhosis who were treated at National Taiwan University Hospital, Taipei, Taiwan, were enrolled. Patients were classified into different groups according to the diagnostic criteria of MAFLD and NAFLD. The clinical and histologic features were then analyzed and compared.
Results: In total, 76 patients (41.1%) were diagnosed with both MAFLD and NAFLD, 81 patients (43.8%) were diagnosed with MAFLD alone, nine patients (4.9%) were diagnosed with NAFLD alone, and 19 patients (10.3%) were diagnosed with neither. Those with MAFLD alone exhibited a higher degree of disease severity regarding histology and laboratory data than those with NAFLD alone. Advanced fibrosis was associated with the presences of hepatitis B virus infection and metabolic diseases.
Conclusions: The novel diagnostic criteria for MAFLD include an additional 38.9% of patients with hepatic steatosis and can better help identify those with a high degree of disease severity for early intervention than can the previous NAFLD criteria.
Keywords: Metabolic dysfunction-associated fatty liver disease, Nonalcoholic fatty liver disease, Metabolic diseases, Hepatic fibrosis, Hepatitis B
Hepatic steatosis has been one of the major etiologies for chronic liver diseases with steadily increasing prevalence especially for those in the urbanized countries.1,2 Unfortunately, in the past years, the international guidelines defined the “nonalcoholic fatty liver disease (NAFLD)” and “nonalcoholic steatohepatitis (NASH)” by excluding other causes of hepatic steatosis, such as alcoholism, pregnancy, hepatitis C virus (HCV) infection and others.3-5 Because several metabolic and chronic diseases are known to be associated with the development of fatty liver disease, the influence and interactions of these chronic or metabolic diseases with hepatic steatosis would not be able to clarify using traditional diagnostic criteria. The expanding criteria of inclusion rather than exclusion are expected to make a more comprehensive overview of the widespread hepatic steatosis disease. A new positive definition for the metabolic dysfunction-associated fatty liver disease (MAFLD) was thus proposed from an international expert consensus in 2020. The diagnosis of MAFLD is made if the patient has hepatic steatosis along with one of the three criteria, namely overweight/obesity, presence of type 2 diabetes mellitus (DM), and at least two metabolic risk abnormalities.6
Since the new definition for MAFLD is a conceptional one, there are debates on the change in terminology,7 and only very limited information about its application in the real world.8 A retrospective study with population-based data from the National Health and Nutrition Examination Surveys in United State during 1988 to 1994 is the only one so far providing the test and validation of the new criteria in clinical practice.8 However, the histologic features were not available in the study. Although a liver biopsy is not required for the diagnosis of MAFLD, provision of liver histology will accurately assess the degree of steatosis as well as the severity of fibrosis, and will be of great help to evaluate the major difference between the prior NAFLD and the novel MAFLD criteria, and the potential clinical impact of new MAFLD diagnostic criteria. Besides, the clinical applications of this new definition in hepatitis B virus (HBV) endemic countries also await further studies to clarify. In this study, we thus aimed to investigate the clinical as well as the histologic features of MAFLD, and compared the NAFLD and MAFLD criteria in patients with biopsy-proven hepatic steatosis.
From January 2009 to December 2019, a total of 780 percutaneous ultrasound-guided liver random biopsies at the National Taiwan University Hospital, Taipei, Taiwan was retrospectively collected. Of these patients, 175 patients with hepatic steatosis and 10 with cryptogenic cirrhosis in the histological reports were identified for evaluation (Fig. 1). This study was approved by the Institutional Review Board of National Taiwan University Hospital (IRB number: NTUH 201909063RINA) and conformed to the ethical guidelines of the 1975 Declaration of Helsinki. The informed consent was waived.
A standardized record form was used for data collection. Retrospective review of the medical records, reports, and images was performed. Age, gender, comorbidity, alcohol consumption, and medication history were recorded. The body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. Laboratory data, such as platelet count, prothrombin time, international normalized ratio, alanine aminotransferas, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, total bilirubin, fasting glucose, hemoglobin A1c, total cholesterol, triglyceride, autoimmune antibodies and the seromarkers for viral hepatitis were collected. The fibrosis-4 score and aspartate aminotransferase to platelet ratio index were also analyzed.
The histologic reports of the patients were recorded, including the grading of steatosis, lobular inflammation and ballooning change according to the NASH Clinical Research Network (NASH CRN). The NAFLD Activity Score (NAS) was calculated.9 The degree of hepatic fibrosis was stratified according to the NASH CRN fibrosis staging system. Hepatic fibrosis with stage 0–2 was defined as early fibrosis while that with stage 3–4 was defined as advanced fibrosis. Specimens with the diagnosis of cryptogenic cirrhosis were reviewed by a consulted liver pathologist additionally.
The diagnosis of MAFLD was made if there were evidence of hepatic steatosis plus one of the following three criteria, namely overweight/obesity (BMI ≥23 kg/m2 in Asians), type 2 DM, or evidence of metabolic dysregulation (≥2 metabolic risk abnormalities as follows: waist circumference ≥90/80 cm in Asian men and women, blood pressure ≥130/85 mm Hg or specific drug treatment, plasma triglycerides ≥150 mg/dL or specific drug treatment, plasma high-density lipoprotein-cholesterol <40 mg/dL for men and <50 mg/dL for women or specific drug treatment, prediabetes, homeostasis model assessment of insulin resistance score ≥2.5, and plasma high-sensitivity C-reactive protein level >2 mg/L).6 The diagnostic criteria for MAFLD-related cirrhosis in those without typical histological steatosis were past or present evidence of metabolic risk factors compatible with the criteria for MAFLD, with documentation of MAFLD on a previous liver biopsy or historical documentation of steatosis by hepatic imaging.6
The diagnosis of NAFLD was made if there was presence of hepatic steatosis >5% of all hepatocytes on the histologic report without the history of excessive alcohol intake (alcohol consumption >140 g per week for men and >70 g per week for women), the usage of steatosis-related medications or chronic liver diseases. The diagnosis of NAFLD-related cirrhosis was made if there was pathological presentation of NAFLD, or with comorbidity of BMI >30 kg/m2, hypertension or type 2 DM, and unexplained by other etiology of chronic liver disease.
In order to demonstrate the difference between the MAFLD and NAFLD criteria, we defined patients meeting the MAFLD but not NAFLD criteria as the “MAFLD-only group,” those meeting the NAFLD rather than MAFLD criteria as the “NAFLD-only group,” and those meeting both MAFLD/NAFLD criteria (Fig. 1). The comparison of the clinical and histologic data was conducted among the three groups and between the MAFLD-only group and NAFLD-only group.
The categorical data was compared by the chi-square and two-tailed Fisher exact tests. The continuous variables were examined by the two-sample t-test. A two-tailed p<0.05 was considered statistically significant. The presences of comorbidities, namely hypertension, type 2 DM, dyslipidemia, HBV infection, HCV infection and alcoholism, were comprehensively included in the logistic regression analyses to determine the association with advanced fibrosis in patients with MAFLD. Comorbidities with p<0.1 in the univariate analyses were used in a multivariate logistic regression model. The statistical analyses were conducted by PASW Statistics for Windows, version 18.0 (SPSS Inc., Chicago, IL, USA).
There were 81 (43.8%) patients fitting the MAFLD criteria alone, nine (4.9%) fitting the NAFLD criteria alone, and 76 (41.1%) fitting the both criteria while 19 (10.3%) not fitting either one (Fig. 1). The indications for liver biopsies in each group are listed in Table 1. In the MAFLD-only group, the reasons for exclusion from NAFLD are demonstrated in Table 2.
The characteristics of the patients are shown in Table 3. Among patients with dual diagnosis (n=76), the percentage of male and female gender were identical. The mean age was 51.26 years and the mean BMI was 27.65 kg/m2. Some patients had comorbidities of cirrhosis (21.1%), hypertension (48.7%), DM (34.2%) and dyslipidemia (40.8%). None had HBV infection, HCV infection and alcoholism because of exclusion by NAFLD criteria. The mean levels of the laboratory data were also demonstrated. The mean fibrosis-4 score was 2.44 while the aspartate aminotransferase to platelet ratio index was 1.90.
The patients meeting only one of the NAFLD or MAFLD criteria were analyzed to compare the difference between the two distinct definitions. There were 81 patients in the MAFLD-only group and nine in the NAFLD-only group. There was no significant difference (p>0.05) in the gender and age, but the BMI was higher in the MAFLD-only group than that in the NAFLD-only group, 27.31 versus 21.36 kg/m2 (p<0.001), respectively. Among the 81 patients in the MAFLD-only group, there were 19 (23.5%) with cirrhosis, 30 (37.0%) with hypertension, 26 (32.1%) with type 2 DM, 16 (19.8%) with dyslipidemia, 42 (51.9%) with HBV infection, 10 (12.3%) with HCV infection, and five (6.2%) with alcoholism. In the NAFLD-only group, there was no patient with above comorbidities. There was no significant difference in the levels of international normalized ratio, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase, fasting glucose, hemoglobin A1c, total cholesterol, triglyceride, fibrosis-4 score and aspartate aminotransferase to platelet ratio index between the two groups. However, the platelet count (198.64×103/μL vs 271.56×103/μL, p=0.010) was significantly lower and the level of total bilirubin (3.09 mg/dL vs 0.70 mg/dL, p=0.002) was significantly higher in the MAFLD-only group than those in the NAFLD-only group.
The histologic degree of steatosis and fibrosis is listed in Table 4. In patients with dual diagnosis, those with steatosis grade 0–1, 2, and 3 were 56.6%, 25.0%, and 18.4%. Those with lobular inflammation grade 0 to 2 were 32.9%, 43.4%, and 23.7%. None had lobular inflammation grade 3. The patients with ballooning change grade 0, 1, and 2–3 were 28.9%, 55.3%, and 15.8%. The mean NAS was 3.38. There were 28 (36.8%) patients with advanced fibrosis according to the NASH CRN system.
The histologic features in the MAFLD-only and NAFLD-only groups were compared. Those with steatosis grade 0–1, 2, and 3 were 65.4%, 23.5%, and 11.1% in the MAFLD-only group, and all the nine patients in the NAFLD-only group had steatosis grade 0–1 (p=0.178). Those with lobular inflammation grade 0 to 2 were 44.4%, 39.5%, and 16.0% in the MAFLD-only group, and 66.7%, 22.2%, and 11.1% in the NAFLD-only group, respectively (p=0.516). None in both groups had lobular inflammation grade 3. The patients with ballooning change grade 0, 1, and 2–3 were 45.7%, 43.2%, and 11.1% in the MAFLD-only group, and 77.8%, 22.2%, and 0.0% in the NAFLD-only group, respectively (p=0.203). The NAS was significantly higher in the MAFLD-only group than that in the NAFLD-only group (2.83 vs 1.67, p=0.009), respectively. There were more patients with advanced fibrosis according to the NASH CRN system in the MAFLD-only group than in the NAFLD-only group, 48.1% versus 0.0% (p=0.005).
In the univariate analysis of association between comorbidities and advanced fibrosis in patients with MAFLD, the presences of hypertension, DM and HBV infection were associated with more advanced fibrosis, while the presence of dyslipidemia was associated with less advanced fibrosis (Table 5). The above comorbidities were then evaluated using a multivariate logistic regression model. Hypertension (odds ratio [OR], 2.051; p=0.047), DM (OR, 2.489; p=0.020) and HBV infection (OR, 2.447; p=0.024) were independently associated with more advanced fibrosis, and dyslipidemia was independently associated with less advanced fibrosis (OR, 0.291; p=0.003).
The newly-proposed definition of MAFLD focuses on the “positive” diagnostic criteria and emphasizes the role and contribution of metabolic dysfunction in fatty liver disease. It is expected to include those with higher risk of disease progression that need specific treatment earlier for the metabolic dysfunction. However, the conceptional criteria for MAFLD have no histologic validation in real world so far, and there is only a study examining the clinical characteristics based on the ultrasonography and laboratory data.8 Liver biopsy, although not performed routinely, remains the gold standard for diagnosis, and is the only reliable method to determine the severity of inflammation and fibrosis accurately.10 This study is the first one to validate the new criteria for MAFLD based on both clinical and histologic information.
The definition of MAFLD is considered to include more patients because the exclusion of alcoholism and chronic liver disease is not required. In our study, the number of patients with MAFLD was greater than those with NAFLD, compatible with the aforementioned expectation. Those with alcoholism, HBV infection and HCV infection excluded by NAFLD criteria were included in the MAFLD group. The concept of concomitant MAFLD and other liver diseases (dual etiology fatty liver disease) was proposed because of the rising global prevalence of metabolic diseases, which may co-exist frequently with other etiologies contributing to hepatic steatosis.11 For example, the presence of HCV infection was reported to be associated with hepatic steatosis and metabolic syndrome.12 In the NAFLD criteria, the exclusion of these patients may lead to underdiagnosis and omit the timely management for the metabolic dysregulation. In our study, HBV infection, hypertension and DM were found to be independently associated with advanced fibrosis in our patients with MAFLD, compatible with the previous studies revealing that the presence of metabolic syndrome or diseases carried a high risk of hepatic fibrosis.13,14 Even in the non-obese populations, those with fatty liver disease had higher risks of DM and metabolic syndrome.15 Those with NAFLD-associated HCC were also known to be usually accompanied with metabolic disorders.16 However, the two comorbidities, HCV and alcoholism, were expected to be associated with advanced fibrosis but not in this study. The plausible explanation may be the small number of these subpopulations for statistical significance. Although the obeticholic acid is a potential candidate of pharmacotherapy for fatty liver disease in the future,17 lifestyle modifications to correct metabolic dysregulation remain the mainstream of treatment.18,19 The new criteria for MAFLD may help to select those with above treatable concurrent conditions.
There were 81 patients not included by the NAFLD criteria but meeting the MAFLD criteria in our study. We compared them with the other nine patients who met the NAFLD criteria but not the MAFLD criteria. The direct comparison helps clarify the major difference between the two distinct disease populations selected by different definitions. The patients in the MAFLD-only group had significantly lower platelet count and higher level of total bilirubin as compared with those in the NAFLD-only group, suggesting that patients with possibly higher degree of disease severity tend to be identified according to the novel MAFLD criteria for further management. In the comparison of histologic features, the NAS and the percentage of patients with advanced fibrosis were also significantly higher in the MAFLD-only group than that in the NAFLD-only group, confirming the nature of higher severity in patients with MAFLD rather than NAFLD by histology. Of note, the nine patients with NAFLD but not MAFLD had a mean NAS of 1.67 only, indicating less disease activity of fatty liver disease.9 Those patients may be classified into so-called “alternative causes” of fatty liver disease in the future according to the consensus statement.6
This study was conducted in the HBV-endemic area and the prevalence reached 26.8% in our patients with MAFLD. Although there are several studies demonstrating the negative correlation between HBV infection and hepatic steatosis,20,21 those with HBV infection were excluded by the diagnostic criteria of NAFLD. In the analysis of factors associated with advanced fibrosis, the presence of HBV infection had a higher risk than the absence in our study. A previous study concluded that concurrent fatty liver proven by histology in HBV patients increases the risk of HCC.22 Another recent study using a large cohort of chronic hepatitis B patients found that those with concomitant NASH and chronic hepatitis B had more advanced fibrosis, mortalities and liver-related adverse outcomes.23 Those with HBV infection and concurrent metabolic factors were known to be associated with an increased risk of HCC than those without.24,25 Since the new criteria focuses on the metabolic dysfunction, it is expected to select more such patients in HBV population for early management to improve the long-term outcomes.
There were several strengths in our study. For the first time, we provide the histologic features and grading of MAFLD in the real world, with emphasis on the difference from NAFLD. In addition, the patients were collected in a recent decade (from 2009 to 2019), which means the baseline characteristics and prevalence of the metabolic diseases were closer to those in current population. Also, the HBV infection was included as a variable for analysis because it was conducted in the HBV-endemic area. There were also some limitations. First, this is a retrospective study conducted in a single tertiary center in Taiwan, and the results need to be validated in the Western countries. Second, the prevalence of MAFLD could not be evaluated in the general population because only patients with histology-proven hepatic steatosis were enrolled for analysis. Third, the number of patients with certain comorbidities, such as alcoholism and HCV, may be too small in our cohort to demonstrate the statistical significance in the correlation with advanced fibrosis. Studies in the future with larger number of biopsy-proven patients may help clarify this point.
In conclusion, the novel diagnostic criteria for MAFLD are useful and help identify patients with higher degree of disease severity and more treatable comorbidities for early intervention.
This study was funded by the Ministry of Science and Technology and Ministry of Health and Welfare, Executive Yuan, Taiwan; National Taiwan University Hospital (NTUH 109-A149), Taipei, Taiwan.
No potential conflict of interest relevant to this article was reported.
Drafting of the article: S.C.H., C.J.L., H.J.S. Data acquisition: H.J.S., S.C.H. Interpretation of the data: C.J.L., S.C.H. Statistical analysis: S.C.H., H.J.S. Conception and design of study: C.J.L., S.C.H., H.J.S. Critical revision for important intellectual content and final approval of the manuscript: S.C.H., C.J.L., H.J.S., J.H.K., T.C.T., H.C.Y., T.H.S., P.J.C. Study supervision: C.J.L., J.H.K., P.J.C.
Indications for Liver Biopsies (n=166)
Indications | Dual diagnosis with MAFLD and NAFLD (n=76) |
MAFLD-only (n=81) |
NAFLD-only (n=9) |
---|---|---|---|
Abnormal liver functions | |||
Hepatocellular injury | 53 (69.7) | 42 (51.9) | 6 (66.7) |
Cholestatic injury | 6 (7.9) | 8 (9.9) | 2 (22.2) |
Hyperbilirubinemia | 3 (3.9) | 10 (12.3) | 0 |
Unknown cirrhosis | 13 (17.1) | 3 (3.7) | 0 |
Evaluation of fibrosis | 0 | 14 (17.3) | 0 |
Hepatic tumor | 1 (1.3) | 4 (4.9) | 1 (11.1) |
Data are presented as number (%).
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease.
Reasons for NAFLD Exclusion in Patients with MAFLD Alone (n=81)
Reasons for exclusion | No. (%) |
---|---|
HBV infection* | 35 (43.2) |
Culprit medications | 23 (28.4) |
HCV infection | 10 (12.3) |
Autoimmune liver diseases | 7 (8.6) |
Alcoholism | 5 (6.2) |
HEV infection | 1 (1.2) |
NAFLD, nonalcoholic fatty liver disease; MAFLD, metabolic dysfunction-associated fatty liver disease; HBV, hepatitis B virus; HCV, hepatitis C virus; HEV, hepatitis E virus.
*Seven patients with concomitant HBV infection were excluded for having other etiologies: three with culprit medications, two with HCV coinfection and two with alcoholism.
Demographics, Characteristics and Laboratory Data (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) |
Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Demographics | ||||
Male sex | 38 (50.0) | 49 (60.5) | 4 (44.4) | 0.479 |
Age, yr | 51.26±15.29 | 51.93±13.44 | 44.11±15.31 | 0.106 |
BMI, kg/m2 | 27.65±4.31 | 27.31±3.58 | 21.36±1.59 | <0.001 |
Comorbidities | ||||
Cirrhosis | 16 (21.1) | 19 (23.5) | 0 | 0.195 |
Hypertension | 37 (48.7) | 30 (37.0) | 0 | 0.027 |
Diabetes mellitus | 26 (34.2) | 26 (32.1) | 0 | 0.055 |
Dyslipidemia | 31 (40.8) | 16 (19.8) | 0 | 0.353 |
HBV infection | 0 | 42 (51.9) | 0 | 0.003 |
HCV infection | 0 | 10 (12.3) | 0 | 0.590 |
Alcoholism | 0 | 5 (6.2) | 0 | 1.000 |
Laboratory data | ||||
Platelet, ×103/μL | 238.67±96.11 | 198.64±81.48 | 271.56±40.36 | 0.010 |
INR | 0.99±0.07 | 1.01±0.09 | 0.97±0.06 | 0.231 |
AST, U/L | 128.38±280.78 | 98.96±91.99 | 107.56±151.48 | 0.805 |
ALT, U/L | 152.79±182.82 | 125.94±147.29 | 139.56±106.52 | 0.789 |
ALP, U/L | 121.37±142.50 | 130.18±162.64 | 121.00±62.52 | 0.868 |
GGT, U/L | 184.51±394.20 | 209.03±310.67 | 108.00±86.91 | 0.338 |
Total bilirubin, mg/dL | 1.59±3.12 | 3.09±6.62 | 0.70±0.29 | 0.002 |
Fasting glucose, mg/dL | 124.02±50.64 | 120.28±48.07 | 118.33±24.50 | 0.945 |
HbA1c, % | 6.37±1.17 | 6.40±1.46 | 5.00±0.00 | 0.345 |
Total cholesterol, mg/dL | 191.73±37.98 | 192.48±96.34 | 208.00±63.17 | 0.786 |
Triglyceride, mg/dL | 161.87±84.85 | 159.24±122.18 | 101.00±41.58 | 0.420 |
FIB-4 score | 2.44±2.50 | 3.02±2.99 | 1.65±2.53 | 0.189 |
APRI | 1.90±4.15 | 1.89±1.83 | 1.48±2.48 | 0.547 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; BMI, body mass index; HBV, hepatitis B virus; HCV, hepatitis C virus; INR, international normalized ratio; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma glutamyl transferase; HbA1c, hemoglobin A1c; FIB-4, fibrosis-4; APRI, aspartate aminotransferase to platelet ratio index.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Histologic Degree of Steatosis and Fibrosis (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) |
Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Steatosis | 0.178 | |||
Grade 0–1 | 43 (56.6) | 53 (65.4) | 9 (100.0) | |
Grade 2 | 19 (25.0) | 19 (23.5) | 0 | |
Grade 3 | 14 (18.4) | 9 (11.1) | 0 | |
Lobular inflammation | 0.516 | |||
Grade 0 | 25 (32.9) | 36 (44.4) | 6 (66.7) | |
Grade 1 | 33 (43.4) | 32 (39.5) | 2 (22.2) | |
Grade 2 | 18 (23.7) | 13 (16.0) | 1 (11.1) | |
Grade 3 | 0 | 0 | 0 | |
Ballooning change | 0.203 | |||
Grade 0 | 22 (28.9) | 37 (45.7) | 7 (77.8) | |
Grade 1 | 42 (55.3) | 35 (43.2) | 2 (22.2) | |
Grade 2–3 | 12 (15.8) | 9 (11.1) | 0 | |
NAS | 3.38±1.63 | 2.83±1.59 | 1.67±1.00 | 0.009 |
Advanced fibrosis (stage 3–4) | 28 (36.8) | 39 (48.1) | 0 | 0.005 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; NAS, NAFLD Activity Score.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Comorbidities Associated with Advanced Fibrosis (Stage 3–4) in Patients with MAFLD (n=157)
Comorbidities | Univariate analysis | Multivariate analysis | |||||
---|---|---|---|---|---|---|---|
OR | 95% CI | p-value | OR | 95% CI | p-value | ||
Hypertension (yes vs no) | 2.210 | 1.156–4.224 | 0.016 | 2.051 | 1.010–4.165 | 0.047 | |
Diabetes mellitus (yes vs no) | 2.223 | 1.130–4.374 | 0.021 | 2.489 | 1.153–5.371 | 0.020 | |
Dyslipidemia (yes vs no) | 0.343 | 0.161–0.729 | 0.005 | 0.291 | 0.128–0.663 | 0.003 | |
HBV infection (yes vs no) | 2.556 | 1.240–5.271 | 0.011 | 2.447 | 1.124–5.325 | 0.024 | |
HCV infection (yes vs no) | 0.889 | 0.241–3.283 | 0.860 | ||||
Alcoholism (yes vs no) | 2.062 | 0.335–12.703 | 0.435 |
MAFLD, metabolic dysfunction-associated fatty liver disease; OR, odds ratio; CI, confidence interval; HBV, hepatitis B virus; HCV, hepatitis C virus.
Gut and Liver 2021; 15(3): 451-458
Published online May 15, 2021 https://doi.org/10.5009/gnl20218
Copyright © Gut and Liver.
Shang-Chin Huang1 , Hau-Jyun Su1
, Jia-Horng Kao1,2,3
, Tai-Chung Tseng1,3
, Hung-Chih Yang1,3
, Tung-Hung Su1,3
, Pei-Jer Chen1,2,3
, Chun-Jen Liu1,2,3
1Department of Internal Medicine, 2Graduate Institute of Clinical Medicine, and 3Hepatitis Research Center, National Taiwan University College of Medicine and Hospital, Taipei City, Taiwan
Correspondence to:Chun-Jen Liu
ORCID https://orcid.org/0000-0002-6202-0993
E-mail cjliu@ntu.edu.tw
Shang-Chin Huang and Hau-Jyun Su contributed equally to this work as first authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background/Aims: Fatty liver disease is defined as a cluster of diseases with heterogeneous etiologies, and its definition continues to evolve. The novel conceptional criteria for metabolic dysfunction-associated fatty liver disease (MAFLD) were proposed in 2020 to avoid the exclusion of a certain subpopulation, but their evaluations have been limited. We aimed to examine and compare the clinical as well as histologic features of MAFLD versus nonalcoholic fatty liver disease (NAFLD) in patients with biopsy-proven hepatic steatosis.
Methods: From January 2009 to December 2019, 175 patients with histology-proven hepatic steatosis and 10 with cryptogenic cirrhosis who were treated at National Taiwan University Hospital, Taipei, Taiwan, were enrolled. Patients were classified into different groups according to the diagnostic criteria of MAFLD and NAFLD. The clinical and histologic features were then analyzed and compared.
Results: In total, 76 patients (41.1%) were diagnosed with both MAFLD and NAFLD, 81 patients (43.8%) were diagnosed with MAFLD alone, nine patients (4.9%) were diagnosed with NAFLD alone, and 19 patients (10.3%) were diagnosed with neither. Those with MAFLD alone exhibited a higher degree of disease severity regarding histology and laboratory data than those with NAFLD alone. Advanced fibrosis was associated with the presences of hepatitis B virus infection and metabolic diseases.
Conclusions: The novel diagnostic criteria for MAFLD include an additional 38.9% of patients with hepatic steatosis and can better help identify those with a high degree of disease severity for early intervention than can the previous NAFLD criteria.
Keywords: Metabolic dysfunction-associated fatty liver disease, Nonalcoholic fatty liver disease, Metabolic diseases, Hepatic fibrosis, Hepatitis B
Hepatic steatosis has been one of the major etiologies for chronic liver diseases with steadily increasing prevalence especially for those in the urbanized countries.1,2 Unfortunately, in the past years, the international guidelines defined the “nonalcoholic fatty liver disease (NAFLD)” and “nonalcoholic steatohepatitis (NASH)” by excluding other causes of hepatic steatosis, such as alcoholism, pregnancy, hepatitis C virus (HCV) infection and others.3-5 Because several metabolic and chronic diseases are known to be associated with the development of fatty liver disease, the influence and interactions of these chronic or metabolic diseases with hepatic steatosis would not be able to clarify using traditional diagnostic criteria. The expanding criteria of inclusion rather than exclusion are expected to make a more comprehensive overview of the widespread hepatic steatosis disease. A new positive definition for the metabolic dysfunction-associated fatty liver disease (MAFLD) was thus proposed from an international expert consensus in 2020. The diagnosis of MAFLD is made if the patient has hepatic steatosis along with one of the three criteria, namely overweight/obesity, presence of type 2 diabetes mellitus (DM), and at least two metabolic risk abnormalities.6
Since the new definition for MAFLD is a conceptional one, there are debates on the change in terminology,7 and only very limited information about its application in the real world.8 A retrospective study with population-based data from the National Health and Nutrition Examination Surveys in United State during 1988 to 1994 is the only one so far providing the test and validation of the new criteria in clinical practice.8 However, the histologic features were not available in the study. Although a liver biopsy is not required for the diagnosis of MAFLD, provision of liver histology will accurately assess the degree of steatosis as well as the severity of fibrosis, and will be of great help to evaluate the major difference between the prior NAFLD and the novel MAFLD criteria, and the potential clinical impact of new MAFLD diagnostic criteria. Besides, the clinical applications of this new definition in hepatitis B virus (HBV) endemic countries also await further studies to clarify. In this study, we thus aimed to investigate the clinical as well as the histologic features of MAFLD, and compared the NAFLD and MAFLD criteria in patients with biopsy-proven hepatic steatosis.
From January 2009 to December 2019, a total of 780 percutaneous ultrasound-guided liver random biopsies at the National Taiwan University Hospital, Taipei, Taiwan was retrospectively collected. Of these patients, 175 patients with hepatic steatosis and 10 with cryptogenic cirrhosis in the histological reports were identified for evaluation (Fig. 1). This study was approved by the Institutional Review Board of National Taiwan University Hospital (IRB number: NTUH 201909063RINA) and conformed to the ethical guidelines of the 1975 Declaration of Helsinki. The informed consent was waived.
A standardized record form was used for data collection. Retrospective review of the medical records, reports, and images was performed. Age, gender, comorbidity, alcohol consumption, and medication history were recorded. The body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. Laboratory data, such as platelet count, prothrombin time, international normalized ratio, alanine aminotransferas, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, total bilirubin, fasting glucose, hemoglobin A1c, total cholesterol, triglyceride, autoimmune antibodies and the seromarkers for viral hepatitis were collected. The fibrosis-4 score and aspartate aminotransferase to platelet ratio index were also analyzed.
The histologic reports of the patients were recorded, including the grading of steatosis, lobular inflammation and ballooning change according to the NASH Clinical Research Network (NASH CRN). The NAFLD Activity Score (NAS) was calculated.9 The degree of hepatic fibrosis was stratified according to the NASH CRN fibrosis staging system. Hepatic fibrosis with stage 0–2 was defined as early fibrosis while that with stage 3–4 was defined as advanced fibrosis. Specimens with the diagnosis of cryptogenic cirrhosis were reviewed by a consulted liver pathologist additionally.
The diagnosis of MAFLD was made if there were evidence of hepatic steatosis plus one of the following three criteria, namely overweight/obesity (BMI ≥23 kg/m2 in Asians), type 2 DM, or evidence of metabolic dysregulation (≥2 metabolic risk abnormalities as follows: waist circumference ≥90/80 cm in Asian men and women, blood pressure ≥130/85 mm Hg or specific drug treatment, plasma triglycerides ≥150 mg/dL or specific drug treatment, plasma high-density lipoprotein-cholesterol <40 mg/dL for men and <50 mg/dL for women or specific drug treatment, prediabetes, homeostasis model assessment of insulin resistance score ≥2.5, and plasma high-sensitivity C-reactive protein level >2 mg/L).6 The diagnostic criteria for MAFLD-related cirrhosis in those without typical histological steatosis were past or present evidence of metabolic risk factors compatible with the criteria for MAFLD, with documentation of MAFLD on a previous liver biopsy or historical documentation of steatosis by hepatic imaging.6
The diagnosis of NAFLD was made if there was presence of hepatic steatosis >5% of all hepatocytes on the histologic report without the history of excessive alcohol intake (alcohol consumption >140 g per week for men and >70 g per week for women), the usage of steatosis-related medications or chronic liver diseases. The diagnosis of NAFLD-related cirrhosis was made if there was pathological presentation of NAFLD, or with comorbidity of BMI >30 kg/m2, hypertension or type 2 DM, and unexplained by other etiology of chronic liver disease.
In order to demonstrate the difference between the MAFLD and NAFLD criteria, we defined patients meeting the MAFLD but not NAFLD criteria as the “MAFLD-only group,” those meeting the NAFLD rather than MAFLD criteria as the “NAFLD-only group,” and those meeting both MAFLD/NAFLD criteria (Fig. 1). The comparison of the clinical and histologic data was conducted among the three groups and between the MAFLD-only group and NAFLD-only group.
The categorical data was compared by the chi-square and two-tailed Fisher exact tests. The continuous variables were examined by the two-sample t-test. A two-tailed p<0.05 was considered statistically significant. The presences of comorbidities, namely hypertension, type 2 DM, dyslipidemia, HBV infection, HCV infection and alcoholism, were comprehensively included in the logistic regression analyses to determine the association with advanced fibrosis in patients with MAFLD. Comorbidities with p<0.1 in the univariate analyses were used in a multivariate logistic regression model. The statistical analyses were conducted by PASW Statistics for Windows, version 18.0 (SPSS Inc., Chicago, IL, USA).
There were 81 (43.8%) patients fitting the MAFLD criteria alone, nine (4.9%) fitting the NAFLD criteria alone, and 76 (41.1%) fitting the both criteria while 19 (10.3%) not fitting either one (Fig. 1). The indications for liver biopsies in each group are listed in Table 1. In the MAFLD-only group, the reasons for exclusion from NAFLD are demonstrated in Table 2.
The characteristics of the patients are shown in Table 3. Among patients with dual diagnosis (n=76), the percentage of male and female gender were identical. The mean age was 51.26 years and the mean BMI was 27.65 kg/m2. Some patients had comorbidities of cirrhosis (21.1%), hypertension (48.7%), DM (34.2%) and dyslipidemia (40.8%). None had HBV infection, HCV infection and alcoholism because of exclusion by NAFLD criteria. The mean levels of the laboratory data were also demonstrated. The mean fibrosis-4 score was 2.44 while the aspartate aminotransferase to platelet ratio index was 1.90.
The patients meeting only one of the NAFLD or MAFLD criteria were analyzed to compare the difference between the two distinct definitions. There were 81 patients in the MAFLD-only group and nine in the NAFLD-only group. There was no significant difference (p>0.05) in the gender and age, but the BMI was higher in the MAFLD-only group than that in the NAFLD-only group, 27.31 versus 21.36 kg/m2 (p<0.001), respectively. Among the 81 patients in the MAFLD-only group, there were 19 (23.5%) with cirrhosis, 30 (37.0%) with hypertension, 26 (32.1%) with type 2 DM, 16 (19.8%) with dyslipidemia, 42 (51.9%) with HBV infection, 10 (12.3%) with HCV infection, and five (6.2%) with alcoholism. In the NAFLD-only group, there was no patient with above comorbidities. There was no significant difference in the levels of international normalized ratio, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase, fasting glucose, hemoglobin A1c, total cholesterol, triglyceride, fibrosis-4 score and aspartate aminotransferase to platelet ratio index between the two groups. However, the platelet count (198.64×103/μL vs 271.56×103/μL, p=0.010) was significantly lower and the level of total bilirubin (3.09 mg/dL vs 0.70 mg/dL, p=0.002) was significantly higher in the MAFLD-only group than those in the NAFLD-only group.
The histologic degree of steatosis and fibrosis is listed in Table 4. In patients with dual diagnosis, those with steatosis grade 0–1, 2, and 3 were 56.6%, 25.0%, and 18.4%. Those with lobular inflammation grade 0 to 2 were 32.9%, 43.4%, and 23.7%. None had lobular inflammation grade 3. The patients with ballooning change grade 0, 1, and 2–3 were 28.9%, 55.3%, and 15.8%. The mean NAS was 3.38. There were 28 (36.8%) patients with advanced fibrosis according to the NASH CRN system.
The histologic features in the MAFLD-only and NAFLD-only groups were compared. Those with steatosis grade 0–1, 2, and 3 were 65.4%, 23.5%, and 11.1% in the MAFLD-only group, and all the nine patients in the NAFLD-only group had steatosis grade 0–1 (p=0.178). Those with lobular inflammation grade 0 to 2 were 44.4%, 39.5%, and 16.0% in the MAFLD-only group, and 66.7%, 22.2%, and 11.1% in the NAFLD-only group, respectively (p=0.516). None in both groups had lobular inflammation grade 3. The patients with ballooning change grade 0, 1, and 2–3 were 45.7%, 43.2%, and 11.1% in the MAFLD-only group, and 77.8%, 22.2%, and 0.0% in the NAFLD-only group, respectively (p=0.203). The NAS was significantly higher in the MAFLD-only group than that in the NAFLD-only group (2.83 vs 1.67, p=0.009), respectively. There were more patients with advanced fibrosis according to the NASH CRN system in the MAFLD-only group than in the NAFLD-only group, 48.1% versus 0.0% (p=0.005).
In the univariate analysis of association between comorbidities and advanced fibrosis in patients with MAFLD, the presences of hypertension, DM and HBV infection were associated with more advanced fibrosis, while the presence of dyslipidemia was associated with less advanced fibrosis (Table 5). The above comorbidities were then evaluated using a multivariate logistic regression model. Hypertension (odds ratio [OR], 2.051; p=0.047), DM (OR, 2.489; p=0.020) and HBV infection (OR, 2.447; p=0.024) were independently associated with more advanced fibrosis, and dyslipidemia was independently associated with less advanced fibrosis (OR, 0.291; p=0.003).
The newly-proposed definition of MAFLD focuses on the “positive” diagnostic criteria and emphasizes the role and contribution of metabolic dysfunction in fatty liver disease. It is expected to include those with higher risk of disease progression that need specific treatment earlier for the metabolic dysfunction. However, the conceptional criteria for MAFLD have no histologic validation in real world so far, and there is only a study examining the clinical characteristics based on the ultrasonography and laboratory data.8 Liver biopsy, although not performed routinely, remains the gold standard for diagnosis, and is the only reliable method to determine the severity of inflammation and fibrosis accurately.10 This study is the first one to validate the new criteria for MAFLD based on both clinical and histologic information.
The definition of MAFLD is considered to include more patients because the exclusion of alcoholism and chronic liver disease is not required. In our study, the number of patients with MAFLD was greater than those with NAFLD, compatible with the aforementioned expectation. Those with alcoholism, HBV infection and HCV infection excluded by NAFLD criteria were included in the MAFLD group. The concept of concomitant MAFLD and other liver diseases (dual etiology fatty liver disease) was proposed because of the rising global prevalence of metabolic diseases, which may co-exist frequently with other etiologies contributing to hepatic steatosis.11 For example, the presence of HCV infection was reported to be associated with hepatic steatosis and metabolic syndrome.12 In the NAFLD criteria, the exclusion of these patients may lead to underdiagnosis and omit the timely management for the metabolic dysregulation. In our study, HBV infection, hypertension and DM were found to be independently associated with advanced fibrosis in our patients with MAFLD, compatible with the previous studies revealing that the presence of metabolic syndrome or diseases carried a high risk of hepatic fibrosis.13,14 Even in the non-obese populations, those with fatty liver disease had higher risks of DM and metabolic syndrome.15 Those with NAFLD-associated HCC were also known to be usually accompanied with metabolic disorders.16 However, the two comorbidities, HCV and alcoholism, were expected to be associated with advanced fibrosis but not in this study. The plausible explanation may be the small number of these subpopulations for statistical significance. Although the obeticholic acid is a potential candidate of pharmacotherapy for fatty liver disease in the future,17 lifestyle modifications to correct metabolic dysregulation remain the mainstream of treatment.18,19 The new criteria for MAFLD may help to select those with above treatable concurrent conditions.
There were 81 patients not included by the NAFLD criteria but meeting the MAFLD criteria in our study. We compared them with the other nine patients who met the NAFLD criteria but not the MAFLD criteria. The direct comparison helps clarify the major difference between the two distinct disease populations selected by different definitions. The patients in the MAFLD-only group had significantly lower platelet count and higher level of total bilirubin as compared with those in the NAFLD-only group, suggesting that patients with possibly higher degree of disease severity tend to be identified according to the novel MAFLD criteria for further management. In the comparison of histologic features, the NAS and the percentage of patients with advanced fibrosis were also significantly higher in the MAFLD-only group than that in the NAFLD-only group, confirming the nature of higher severity in patients with MAFLD rather than NAFLD by histology. Of note, the nine patients with NAFLD but not MAFLD had a mean NAS of 1.67 only, indicating less disease activity of fatty liver disease.9 Those patients may be classified into so-called “alternative causes” of fatty liver disease in the future according to the consensus statement.6
This study was conducted in the HBV-endemic area and the prevalence reached 26.8% in our patients with MAFLD. Although there are several studies demonstrating the negative correlation between HBV infection and hepatic steatosis,20,21 those with HBV infection were excluded by the diagnostic criteria of NAFLD. In the analysis of factors associated with advanced fibrosis, the presence of HBV infection had a higher risk than the absence in our study. A previous study concluded that concurrent fatty liver proven by histology in HBV patients increases the risk of HCC.22 Another recent study using a large cohort of chronic hepatitis B patients found that those with concomitant NASH and chronic hepatitis B had more advanced fibrosis, mortalities and liver-related adverse outcomes.23 Those with HBV infection and concurrent metabolic factors were known to be associated with an increased risk of HCC than those without.24,25 Since the new criteria focuses on the metabolic dysfunction, it is expected to select more such patients in HBV population for early management to improve the long-term outcomes.
There were several strengths in our study. For the first time, we provide the histologic features and grading of MAFLD in the real world, with emphasis on the difference from NAFLD. In addition, the patients were collected in a recent decade (from 2009 to 2019), which means the baseline characteristics and prevalence of the metabolic diseases were closer to those in current population. Also, the HBV infection was included as a variable for analysis because it was conducted in the HBV-endemic area. There were also some limitations. First, this is a retrospective study conducted in a single tertiary center in Taiwan, and the results need to be validated in the Western countries. Second, the prevalence of MAFLD could not be evaluated in the general population because only patients with histology-proven hepatic steatosis were enrolled for analysis. Third, the number of patients with certain comorbidities, such as alcoholism and HCV, may be too small in our cohort to demonstrate the statistical significance in the correlation with advanced fibrosis. Studies in the future with larger number of biopsy-proven patients may help clarify this point.
In conclusion, the novel diagnostic criteria for MAFLD are useful and help identify patients with higher degree of disease severity and more treatable comorbidities for early intervention.
This study was funded by the Ministry of Science and Technology and Ministry of Health and Welfare, Executive Yuan, Taiwan; National Taiwan University Hospital (NTUH 109-A149), Taipei, Taiwan.
No potential conflict of interest relevant to this article was reported.
Drafting of the article: S.C.H., C.J.L., H.J.S. Data acquisition: H.J.S., S.C.H. Interpretation of the data: C.J.L., S.C.H. Statistical analysis: S.C.H., H.J.S. Conception and design of study: C.J.L., S.C.H., H.J.S. Critical revision for important intellectual content and final approval of the manuscript: S.C.H., C.J.L., H.J.S., J.H.K., T.C.T., H.C.Y., T.H.S., P.J.C. Study supervision: C.J.L., J.H.K., P.J.C.
Indications for Liver Biopsies (n=166)
Indications | Dual diagnosis with MAFLD and NAFLD (n=76) |
MAFLD-only (n=81) |
NAFLD-only (n=9) |
---|---|---|---|
Abnormal liver functions | |||
Hepatocellular injury | 53 (69.7) | 42 (51.9) | 6 (66.7) |
Cholestatic injury | 6 (7.9) | 8 (9.9) | 2 (22.2) |
Hyperbilirubinemia | 3 (3.9) | 10 (12.3) | 0 |
Unknown cirrhosis | 13 (17.1) | 3 (3.7) | 0 |
Evaluation of fibrosis | 0 | 14 (17.3) | 0 |
Hepatic tumor | 1 (1.3) | 4 (4.9) | 1 (11.1) |
Data are presented as number (%).
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease.
Reasons for NAFLD Exclusion in Patients with MAFLD Alone (n=81)
Reasons for exclusion | No. (%) |
---|---|
HBV infection* | 35 (43.2) |
Culprit medications | 23 (28.4) |
HCV infection | 10 (12.3) |
Autoimmune liver diseases | 7 (8.6) |
Alcoholism | 5 (6.2) |
HEV infection | 1 (1.2) |
NAFLD, nonalcoholic fatty liver disease; MAFLD, metabolic dysfunction-associated fatty liver disease; HBV, hepatitis B virus; HCV, hepatitis C virus; HEV, hepatitis E virus.
*Seven patients with concomitant HBV infection were excluded for having other etiologies: three with culprit medications, two with HCV coinfection and two with alcoholism.
Demographics, Characteristics and Laboratory Data (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) |
Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Demographics | ||||
Male sex | 38 (50.0) | 49 (60.5) | 4 (44.4) | 0.479 |
Age, yr | 51.26±15.29 | 51.93±13.44 | 44.11±15.31 | 0.106 |
BMI, kg/m2 | 27.65±4.31 | 27.31±3.58 | 21.36±1.59 | <0.001 |
Comorbidities | ||||
Cirrhosis | 16 (21.1) | 19 (23.5) | 0 | 0.195 |
Hypertension | 37 (48.7) | 30 (37.0) | 0 | 0.027 |
Diabetes mellitus | 26 (34.2) | 26 (32.1) | 0 | 0.055 |
Dyslipidemia | 31 (40.8) | 16 (19.8) | 0 | 0.353 |
HBV infection | 0 | 42 (51.9) | 0 | 0.003 |
HCV infection | 0 | 10 (12.3) | 0 | 0.590 |
Alcoholism | 0 | 5 (6.2) | 0 | 1.000 |
Laboratory data | ||||
Platelet, ×103/μL | 238.67±96.11 | 198.64±81.48 | 271.56±40.36 | 0.010 |
INR | 0.99±0.07 | 1.01±0.09 | 0.97±0.06 | 0.231 |
AST, U/L | 128.38±280.78 | 98.96±91.99 | 107.56±151.48 | 0.805 |
ALT, U/L | 152.79±182.82 | 125.94±147.29 | 139.56±106.52 | 0.789 |
ALP, U/L | 121.37±142.50 | 130.18±162.64 | 121.00±62.52 | 0.868 |
GGT, U/L | 184.51±394.20 | 209.03±310.67 | 108.00±86.91 | 0.338 |
Total bilirubin, mg/dL | 1.59±3.12 | 3.09±6.62 | 0.70±0.29 | 0.002 |
Fasting glucose, mg/dL | 124.02±50.64 | 120.28±48.07 | 118.33±24.50 | 0.945 |
HbA1c, % | 6.37±1.17 | 6.40±1.46 | 5.00±0.00 | 0.345 |
Total cholesterol, mg/dL | 191.73±37.98 | 192.48±96.34 | 208.00±63.17 | 0.786 |
Triglyceride, mg/dL | 161.87±84.85 | 159.24±122.18 | 101.00±41.58 | 0.420 |
FIB-4 score | 2.44±2.50 | 3.02±2.99 | 1.65±2.53 | 0.189 |
APRI | 1.90±4.15 | 1.89±1.83 | 1.48±2.48 | 0.547 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; BMI, body mass index; HBV, hepatitis B virus; HCV, hepatitis C virus; INR, international normalized ratio; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma glutamyl transferase; HbA1c, hemoglobin A1c; FIB-4, fibrosis-4; APRI, aspartate aminotransferase to platelet ratio index.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Histologic Degree of Steatosis and Fibrosis (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) |
Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Steatosis | 0.178 | |||
Grade 0–1 | 43 (56.6) | 53 (65.4) | 9 (100.0) | |
Grade 2 | 19 (25.0) | 19 (23.5) | 0 | |
Grade 3 | 14 (18.4) | 9 (11.1) | 0 | |
Lobular inflammation | 0.516 | |||
Grade 0 | 25 (32.9) | 36 (44.4) | 6 (66.7) | |
Grade 1 | 33 (43.4) | 32 (39.5) | 2 (22.2) | |
Grade 2 | 18 (23.7) | 13 (16.0) | 1 (11.1) | |
Grade 3 | 0 | 0 | 0 | |
Ballooning change | 0.203 | |||
Grade 0 | 22 (28.9) | 37 (45.7) | 7 (77.8) | |
Grade 1 | 42 (55.3) | 35 (43.2) | 2 (22.2) | |
Grade 2–3 | 12 (15.8) | 9 (11.1) | 0 | |
NAS | 3.38±1.63 | 2.83±1.59 | 1.67±1.00 | 0.009 |
Advanced fibrosis (stage 3–4) | 28 (36.8) | 39 (48.1) | 0 | 0.005 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; NAS, NAFLD Activity Score.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Comorbidities Associated with Advanced Fibrosis (Stage 3–4) in Patients with MAFLD (n=157)
Comorbidities | Univariate analysis | Multivariate analysis | |||||
---|---|---|---|---|---|---|---|
OR | 95% CI | p-value | OR | 95% CI | p-value | ||
Hypertension (yes vs no) | 2.210 | 1.156–4.224 | 0.016 | 2.051 | 1.010–4.165 | 0.047 | |
Diabetes mellitus (yes vs no) | 2.223 | 1.130–4.374 | 0.021 | 2.489 | 1.153–5.371 | 0.020 | |
Dyslipidemia (yes vs no) | 0.343 | 0.161–0.729 | 0.005 | 0.291 | 0.128–0.663 | 0.003 | |
HBV infection (yes vs no) | 2.556 | 1.240–5.271 | 0.011 | 2.447 | 1.124–5.325 | 0.024 | |
HCV infection (yes vs no) | 0.889 | 0.241–3.283 | 0.860 | ||||
Alcoholism (yes vs no) | 2.062 | 0.335–12.703 | 0.435 |
MAFLD, metabolic dysfunction-associated fatty liver disease; OR, odds ratio; CI, confidence interval; HBV, hepatitis B virus; HCV, hepatitis C virus.
Table 1 Indications for Liver Biopsies (n=166)
Indications | Dual diagnosis with MAFLD and NAFLD (n=76) | MAFLD-only (n=81) | NAFLD-only (n=9) |
---|---|---|---|
Abnormal liver functions | |||
Hepatocellular injury | 53 (69.7) | 42 (51.9) | 6 (66.7) |
Cholestatic injury | 6 (7.9) | 8 (9.9) | 2 (22.2) |
Hyperbilirubinemia | 3 (3.9) | 10 (12.3) | 0 |
Unknown cirrhosis | 13 (17.1) | 3 (3.7) | 0 |
Evaluation of fibrosis | 0 | 14 (17.3) | 0 |
Hepatic tumor | 1 (1.3) | 4 (4.9) | 1 (11.1) |
Data are presented as number (%).
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease.
Table 2 Reasons for NAFLD Exclusion in Patients with MAFLD Alone (n=81)
Reasons for exclusion | No. (%) |
---|---|
HBV infection* | 35 (43.2) |
Culprit medications | 23 (28.4) |
HCV infection | 10 (12.3) |
Autoimmune liver diseases | 7 (8.6) |
Alcoholism | 5 (6.2) |
HEV infection | 1 (1.2) |
NAFLD, nonalcoholic fatty liver disease; MAFLD, metabolic dysfunction-associated fatty liver disease; HBV, hepatitis B virus; HCV, hepatitis C virus; HEV, hepatitis E virus.
*Seven patients with concomitant HBV infection were excluded for having other etiologies: three with culprit medications, two with HCV coinfection and two with alcoholism.
Table 3 Demographics, Characteristics and Laboratory Data (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) | Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Demographics | ||||
Male sex | 38 (50.0) | 49 (60.5) | 4 (44.4) | 0.479 |
Age, yr | 51.26±15.29 | 51.93±13.44 | 44.11±15.31 | 0.106 |
BMI, kg/m2 | 27.65±4.31 | 27.31±3.58 | 21.36±1.59 | <0.001 |
Comorbidities | ||||
Cirrhosis | 16 (21.1) | 19 (23.5) | 0 | 0.195 |
Hypertension | 37 (48.7) | 30 (37.0) | 0 | 0.027 |
Diabetes mellitus | 26 (34.2) | 26 (32.1) | 0 | 0.055 |
Dyslipidemia | 31 (40.8) | 16 (19.8) | 0 | 0.353 |
HBV infection | 0 | 42 (51.9) | 0 | 0.003 |
HCV infection | 0 | 10 (12.3) | 0 | 0.590 |
Alcoholism | 0 | 5 (6.2) | 0 | 1.000 |
Laboratory data | ||||
Platelet, ×103/μL | 238.67±96.11 | 198.64±81.48 | 271.56±40.36 | 0.010 |
INR | 0.99±0.07 | 1.01±0.09 | 0.97±0.06 | 0.231 |
AST, U/L | 128.38±280.78 | 98.96±91.99 | 107.56±151.48 | 0.805 |
ALT, U/L | 152.79±182.82 | 125.94±147.29 | 139.56±106.52 | 0.789 |
ALP, U/L | 121.37±142.50 | 130.18±162.64 | 121.00±62.52 | 0.868 |
GGT, U/L | 184.51±394.20 | 209.03±310.67 | 108.00±86.91 | 0.338 |
Total bilirubin, mg/dL | 1.59±3.12 | 3.09±6.62 | 0.70±0.29 | 0.002 |
Fasting glucose, mg/dL | 124.02±50.64 | 120.28±48.07 | 118.33±24.50 | 0.945 |
HbA1c, % | 6.37±1.17 | 6.40±1.46 | 5.00±0.00 | 0.345 |
Total cholesterol, mg/dL | 191.73±37.98 | 192.48±96.34 | 208.00±63.17 | 0.786 |
Triglyceride, mg/dL | 161.87±84.85 | 159.24±122.18 | 101.00±41.58 | 0.420 |
FIB-4 score | 2.44±2.50 | 3.02±2.99 | 1.65±2.53 | 0.189 |
APRI | 1.90±4.15 | 1.89±1.83 | 1.48±2.48 | 0.547 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; BMI, body mass index; HBV, hepatitis B virus; HCV, hepatitis C virus; INR, international normalized ratio; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma glutamyl transferase; HbA1c, hemoglobin A1c; FIB-4, fibrosis-4; APRI, aspartate aminotransferase to platelet ratio index.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Table 4 Histologic Degree of Steatosis and Fibrosis (n=166)
Variable | Dual diagnosis with MAFLD and NAFLD (n=76) | Single diagnosis | ||
---|---|---|---|---|
MAFLD-only (n=81) | NAFLD-only (n=9) | p-value* | ||
Steatosis | 0.178 | |||
Grade 0–1 | 43 (56.6) | 53 (65.4) | 9 (100.0) | |
Grade 2 | 19 (25.0) | 19 (23.5) | 0 | |
Grade 3 | 14 (18.4) | 9 (11.1) | 0 | |
Lobular inflammation | 0.516 | |||
Grade 0 | 25 (32.9) | 36 (44.4) | 6 (66.7) | |
Grade 1 | 33 (43.4) | 32 (39.5) | 2 (22.2) | |
Grade 2 | 18 (23.7) | 13 (16.0) | 1 (11.1) | |
Grade 3 | 0 | 0 | 0 | |
Ballooning change | 0.203 | |||
Grade 0 | 22 (28.9) | 37 (45.7) | 7 (77.8) | |
Grade 1 | 42 (55.3) | 35 (43.2) | 2 (22.2) | |
Grade 2–3 | 12 (15.8) | 9 (11.1) | 0 | |
NAS | 3.38±1.63 | 2.83±1.59 | 1.67±1.00 | 0.009 |
Advanced fibrosis (stage 3–4) | 28 (36.8) | 39 (48.1) | 0 | 0.005 |
Data are presented as number (%) or mean±SD.
MAFLD, metabolic dysfunction-associated fatty liver disease; NAFLD, nonalcoholic fatty liver disease; NAS, NAFLD Activity Score.
*The statistical analysis and comparison were conducted between the MAFLD-only and NAFLD-only groups.
Table 5 Comorbidities Associated with Advanced Fibrosis (Stage 3–4) in Patients with MAFLD (n=157)
Comorbidities | Univariate analysis | Multivariate analysis | |||||
---|---|---|---|---|---|---|---|
OR | 95% CI | p-value | OR | 95% CI | p-value | ||
Hypertension (yes vs no) | 2.210 | 1.156–4.224 | 0.016 | 2.051 | 1.010–4.165 | 0.047 | |
Diabetes mellitus (yes vs no) | 2.223 | 1.130–4.374 | 0.021 | 2.489 | 1.153–5.371 | 0.020 | |
Dyslipidemia (yes vs no) | 0.343 | 0.161–0.729 | 0.005 | 0.291 | 0.128–0.663 | 0.003 | |
HBV infection (yes vs no) | 2.556 | 1.240–5.271 | 0.011 | 2.447 | 1.124–5.325 | 0.024 | |
HCV infection (yes vs no) | 0.889 | 0.241–3.283 | 0.860 | ||||
Alcoholism (yes vs no) | 2.062 | 0.335–12.703 | 0.435 |
MAFLD, metabolic dysfunction-associated fatty liver disease; OR, odds ratio; CI, confidence interval; HBV, hepatitis B virus; HCV, hepatitis C virus.