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Fig. 2. Differences in apical sodium-dependent bile acid transporter (ASBT) expression in small bile ducts (SBDs) and large bile ducts (LBDs). (A) Immunohistochemical (IHC) showed ASBT expression in the apical membrane of cholangiocytes (×400). (B) With decreasing bile duct diameter, the ASBT expression level gradually decreased, and almost no ASBT expression was observed in the small periportal bile ducts (IHC, ×400). (C) Based on the IHC images, quantification of ASBT expression was performed by Image-Pro Plus v 6.0. (D) Quantitative real-time polymerase chain reaction (qRT-PCR) showed significantly higher ASBT mRNA expression level in the LBDs than in the SBDs. (E) After PCR analysis, the amplified PCR products were identified by electrophoresis, and (F) Western blotting results also showed a significantly higher ASBT protein expression level in the LBDs than in the SBDs. (G) Quantification of the ASBT protein band was performed and normalized relative to the expression level of β-actin. *p<0.05: SBDs versus LBDs.
Gut and Liver 2019;13:569~575 https://doi.org/10.5009/gnl18265
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